Cryopreservation (Egg/Sperm/Embryo)

Cryopreservation is an innovative technique used to freeze eggs, embryos and sperm for use in ART procedures like IUI, IVF & ICSI in future. If the embryos are frozen, a woman doesn’t require a stimulation process for the next infertility treatment cycle.

The cryopreservation of embryos and sperm have become conventional methods in these modern days for assisted reproduction. Similarly, the cryopreservation of oocytes in clinical practice is gaining more popularity and being used widely. 

Embryo cryopreservation has maximized the effectiveness of ART procedures, particularly IVF cycles. The statistical data shows that the birth rate has improved to 8% in infertile women who obtained pregnancy using their fresh and frozen embryos. Cryopreservation Oocytes have even more benefits than Embryo Vitrification.

Egg Vitrification

Egg Vitrification is a flash-freezing procedure to preserve the eggs of women collected during an ART procedure or to postpone pregnancy. It might be due to medical reasons or social reasons. However, over time, your fertility decreases due to the ageing of your ovaries as you become old. Therefore, it becomes difficult for a woman to conceive and have a healthy baby.

The main benefit of freezing your egg is that the qualities present in you at that particular age will be locked. Therefore, this can give you a better chance to have a successful and healthy pregnancy in future by using your eggs when frozen when you were young… Read More Detailed Information

Embryo Vitrification

At ZIVA, we offer a new rapid cryopreservation technique called “vitrification”. Vitrification is a technology that freezes your embryo swiftly to -196ºC, where they become “glass-like” or “vitrified.” You can use the frozen embryo later in future. It freezes the embryo rapidly so that there is no crystal formation. Therefore, it limits the damage during the thawing process. Embryo freezing can be used for both medical and social fertility preservation.

Embryos can be frozen at all stages of their development, from a zygote (fertilized ovum) through its cleavage state (2- 16 cells) to blastocyst (5-6 days). Thus, vitrification is an effective method used in the laboratory to provide successful pregnancy results.

As a part of your IVF cycle, your embryo can be preserved by vitrification. During your IVF procedure, when there are excess embryos that are good in their quality, they can be frozen. Then, by monitoring your natural cycle for ovulation, the embryo can be thawed and implanted timely.

How does it work?

  • The embryos will be kept in a preservative known as a cryo-protectant and frozen with the help of liquid nitrogen.
  • We then rapidly cool down the cryoprotectant containing the embryo to -196 degrees Celsius.
  • There is no ice formation, and the embryo will be stored in a sealed case. 
  • Once you wish to have the embryo transferred, they are rapidly thawed first. 
  • After a few hours, the transfer will take place.

Embryo freezing includes the complete IVF cycle and freezing the embryos…Read More Detailed Information

Sperm Banking

Sperm banking is the process of collecting and storing sperm in extremely low temperatures. Sperm banking is technically called sperm cryopreservation or semen storage. In andrology services, ZIVA is providing sperm freezing, also known as semen cryopreservation. It is a highly effective technique where the functions and sperm motility are also preserved. In Assisted Reproductive Technology (ART), storage of sperm is usually done for future use.

Sperm cryopreservation is done in situations like:

  • Before undergoing chemotherapy or radiation therapy
  • Testicular surgery or vasectomy
  • Exposure to chemicals or extreme heat conditions.
  • Low sperm count or low-quality sperm

How are sperms collected and frozen?

  • Your semen sample is collected through a natural ejaculation process in a small sterile container at the clinic
  • If sperms are not present in the semen sample, then they are surgically retrieved
  • Once the sample is collected, it is tested for its quality, motility and morphology
  • The sperm sample is then preserved in a cryo-protectant in small tubes.
  • The frozen sample is stored in the lab until required.
  • When required, the sample is thawed, washed and tested for mobility
  • It is then used in IUI or IVF treatment.

Cryopreservation is evolving rapidly because of its benefits… Read More Detailed Information